Characterization of zebrafish larvae with knockouts in the NMDA receptor subunit genes grin2Aa and grin2Ab
Vera Abramova, Bohdan Kysilov, Ales Balik, Eni Tomovic, Tereza Smejkalova, Miloslav Korinek, Klevinda Fili, Mark Dobrovolskii, Paulina Bozikova, Jiri Cerny, Ladislav Vyklicky · FENS Forum 2024
Danio rerio (zebrafish) is a model for studying neurophysiological development and gene mutation consequences. Zebrafish possesses orthologs of human NMDA receptor subunit genes, including grin2Aa and grin2Ab for GRIN2A. The grin2Ab knockout strain created via CRISPR-Cas9, and the grin2Aa knockout were investigated for spatial and temporal gene expression and swimming behavior. Whole-mount in situ hybridization (WISH) was performed on wild-type (WT) zebrafish larvae aged 1, 3, and 5 days post fertilization (dpf). The results indicated minimal expression of both genes at 1 dpf. By 3 dpf, grin2A
ePoster · Neuroscience