Topic: guanine deaminase

Cypin (cytosolic PSD-95 interactor) is the most highly expressed guanine deaminase in the brain. Cypin regulates neuronal development by increasing dendritogenesis, modifying dendritic spine morphology, and protecting neurons from injury. To identify binding partners to cypin that may act to mediate these functions, we performed a yeast two-hybrid screen of a rat brain cDNA library with cypin as bait. One of the interactors identified is the beta 7 subunit of the proteasome. Using ubiquitin-independent and ubiquitin-dependent sensors in cultured cells, we found that cypin acts via both pathway