Topic: HET0016

Introduction: Neurovascular coupling (NVC) becomes impaired upon acute ischemic stroke (AIS). NVC dysfunction is thought to be linked to the evolution of spreading depolarization (SD), that causes vasoconstriction by augmenting the synthesis of 20-HETE. Here, we demonstrate the SD-related disruption of NVC during reperfusion, and block 20-HETE production to improve NVC.Methods: C57BL/6 mice (n=41) were anesthetized with isoflurane. Cerebral blood flow (CBF) variations were captured using laser speckle contrast imaging (LASCA) or laser-Doppler flowmetry. After 10 min baseline recording, transie