Characterizing human-derived neuronal network using high-density MEAs and proteomics: In-vitro model for neurodevelopmental disease
Lorenzo Muzzi, Ilaria Musante, Simona Baldassari, Martina Bortolucci, Niccolò Callegari, Andrea Petretto, Federico Zara, Paolo Scudieri · FENS Forum 2024
While dissociated cultures of rodent neurons on microelectrode arrays (MEAs) have become a prominent method for studying neuronal network properties, establishing human neuronal cultures with mature electrophysiological features compatible with MEAs has posed a significant challenge. The aim of this study was to establish a reliable protocol for generating human-derived neuronal networks on High Density Microelectrode Arrays (HD-MEA) and to characterize their electrophysiological features for potential applications in disease modeling and therapeutic testing. Human-induced pluripotent stem cel
ePoster · Neuroscience