Evaluating the effect of astrocyte-derived cholesterol on oligodendroglial maturation in an in vitro model of hypoxic brain injury
Vadanya Shrivastava, Devanjan Dey, Sagar Tyagi, Archna Singh, Jai Bhagwan Sharma, Jayanth Kumar Palanichamy, Pankaj Seth, Sudip Sen · FENS Forum 2024
In preterm infants, hypoxic injury to premyelinating oligodendrocytes leads to failure of oligodendroglial maturation causing long-term neuromotor disabilities. As astrocytes influence oligodendrocyte maturation, this study aimed to analyze the transcriptomic changes in astrocytes after hypoxic injury, to identify possible pathways influencing myelination. Human fetal neural stem cell-derived astrocytes, expressing astrocyte-specific glial fibrillary-acidic protein (GFAP) and excitatory amino acid transporters (EAAT1 and EAAT2), were exposed to hypoxia (0.2% oxygen) and normoxia (20% oxygen) f
ePoster · Neuroscience