Probing neural circuit motifs in zebrafish using holographic optogenetics
Rahul Trivedi, Jennifer Li, Drew Robson · FENS Forum 2024
Two-photon Computer Generated Holography (CGH) based optogenetics allows activation of arbitrarily selected neurons simultaneously in 3D with cellular precision. We integrate two-photon CGH with a single-photon, dual channel light sheet microscope to record whole-brain Ca2+ activity at up to 400 frames per second, for concurrent activation and imaging. We use transgenic zebrafish larvae (5-8 days post fertilization) pan-neuronally expressing an excitatory opsin (HuC:ReaChR) and a Ca2+ indicator (HuC:GCaMP8s). This opsin-indicator combination enables flexible, simultaneous read/write access to
ePoster · Neuroscience