Effect of different fixation protocols on human brain tissue preservation and immunogenicity
Bibiána Török, Katalin Zsófia Tóth, Cecília Szekeres-Paraczky, Péter Szocsics, Zsófia Maglóczky · FENS Forum 2024
The effect of post mortem delay, age of the subjects and fixation protocols were examined on immunostaining of the human brain samples.Short post-mortem time (2-5h) is crucial for the quality of human brain samples. Our group uses cerebral cortical samples and hippocampi with this short post-mortem period, which are suitable for fine structural research. Here the advantages and disadvantages of perfusion and immersion fixation were investigated, and the quality of different immunohistochemical stainings (calbindin, calretinin, parvalbumin, nonphosphorylated neurofilament H /SMI32/ among others
ePoster · Neuroscience