Topic: human cortical neuronal networks

Neuronal networks derived from human induced pluripotent stem cells (hiPSCs) have gained attention for their potential use in in vitro modeling of epilepsy and seizure-like electrophysiological activity. Similar to in vivo animal models, seizure-like activity can be induced in these neuronal cultures using kainic acid (KA), direct agonist of the glutamate receptors. However, to establish more relevant in vitro model using KA, better understanding of KA mediated acute and prolonged effects on human neuronal networks is required. In this study, we investigated both acute and long-term KA concent