Topic: human-induced pluripotent stem cells

While dissociated cultures of rodent neurons on microelectrode arrays (MEAs) have become a prominent method for studying neuronal network properties, establishing human neuronal cultures with mature electrophysiological features compatible with MEAs has posed a significant challenge. The aim of this study was to establish a reliable protocol for generating human-derived neuronal networks on High Density Microelectrode Arrays (HD-MEA) and to characterize their electrophysiological features for potential applications in disease modeling and therapeutic testing. Human-induced pluripotent stem cel

Our research focuses on the elucidation of the PRPF31 gene mutation's impact on retinal cells, specifically within the context of Retinitis Pigmentosa (RP), employing human-induced pluripotent stem cells (hiPSCs). These hiPSCs, sourced from a patient with severe RP harboring the PRPF31 mutation and a healthy control, were differentiated into retinal organoids (ROs) using a refined protocol. The study's methodology encompassed a range of techniques including flow cytometry for assessing pluripotency, immunofluorescence and qPCR for progenitor and photoreceptor-specific markers, microelectrode a