Topic: Human organotypic culture

To understand the human brain and its neurodegenerative diseases, as well as to develop appropriate treatments, we need to investigate human tissue. Human organotypic culture provides an excellent opportunity to examine cellular and network-level activities, as well as molecular changes at long-term, as this technique enables the maintenance of the brain tissue for several weeks with a preserved tissue structure and cellular connections. However, due to the slice preparation and culture technique, considerable changes occur in the cultured tissue. In this study, we explored neuronal degenerati