Topic: human stem cell sources

Disorders of the brain are often therapeutically managed rather than curative with the number of treatment modalities stalling in recent years. There is a lack of relevant drug screening models because current in vitro 2D cell culture systems fail to recapitulate adult neuronal network connectivity [1]. Here, we culture 3D neuronal networks displaying a level of functional connectivity and maturation which is not achieved using standard culture techniques.Polymer scaffolds were 3D printed using a digital light processor with a microstructure resolution of ~50μm and a maximum structure size of