High sensitivity mapping of brain-wide functional networks in awake mice using simultaneous multi-slice fUS imaging
Jeremy Ferrier, Adrien Bertolo, Silvia Cazzanelli, Samuel Diebolt, Sophie Pezet, Mickael Tanter, Bruno-Felix Osmanski, Mathieu Pernot, Thomas Deffieux · FENS Forum 2024
Functional ultrasound (fUS) imaging has recently allowed 3D functional imaging in awake mice using motorized linear or matrix arrays (Brunner et al., 2020; Bertolo et al., 2021). However, the tradeoff between the field of view and temporal resolution introduced by motorized scanning hinders acquiring whole-brain resting state Functional Connectivity (rsFC), whereas the limited sensitivity of matrix arrays prevents transcranial imaging in mice. Here we propose a new hybrid approach dedicated to 3D rsFC in the whole mouse brain with intact skull.A newly developed 15 MHz multiarray probe (IcoPrim
ePoster · Neuroscience