Functional and behavioral characterization of mice carrying a frameshift variant in the NMDA receptor GluN2B subunit
Klevinda Fili, Viktor Kuchtiak, Miriam Candelas Serra, Agnieszka Kubik Zahorodna, Ales Balik, Tereza Smejkalova, Ladislav Vyklicky · FENS Forum 2024
N-methyl-D-aspartate receptor (NMDAR) is a glutamate-gated ion channel permeable to calcium that plays critical roles in synaptic transmission, synaptogenesis, and plasticity. Recent sequencing data in neurological and psychiatric patients reveal numerous variants in genes encoding NMDAR subunits. In our study, we have created and evaluated a transgenic mouse line carrying a frameshift variant (Grin2b L825FfsX4) resulting in the deletion of about half of the GluN2B subunit. Our methods included patch-clamp recordings from hippocampal neurons in both wild-type (Grin2bWT/WT) and transgenic (Grin
ePoster · Neuroscience