Topic: iGABASnFR2

The iGABASnFR is a genetically encoded optical sensor that can detect the inhibitory neurotransmitter GABA. Its recent improvements have allowed it to reliably monitor GABA release evoked by electric stimulation (Marvin et al., Nature Methods 2019) or epileptiform activity (Magloire et al., Curr Biol. 2023). Here, we employed iGABASnFR2, both neuronal and astrocytic variants, virally expressed in the hippocampus or somatosensory cortical region for ~4 weeks of in vivo transduction for high-resolution multiplexed two-photon excitation imaging in acute hippocampal slices or in the barrel cortex