Investigating gut-microbe interactions and epithelial α-synuclein through human enteroid monolayers and imaging flow cytometry of enteroendocrine cells in vitro
Anastazja Gorecki, Chidozie Anyaegbu, Varsha Singh, Kathryn Fuller, Subhash Kulkarni, Ryan Anderton · FENS Forum 2024
Pro-inflammatory environmental factors and disruptions to the gut microbiome are recognised contributors to Parkinson’s disease, and exogenous α-synuclein fibrils spread from the gut to brain via the vagus nerve in animal models. However, the natural cause of enteric α-synuclein pathology is unknown, but emerging research implicates enteroendocrine cells in the gut epithelium. We investigated epithelial α-synuclein through confocal microscopy and western blotting of human intestinal epithelial monolayers (Aim 1), and imaging flow cytometry of murine enteroendocrine STC-1 cells (Aim 2). Aim 1 d
ePoster · Neuroscience