Topic: Ingenuity Pathway Analysis

Increased levels of TNF-Related Weak Inducer of Apoptosis (TWEAK) are associated with neuroinflammation, especially during multiple sclerosis (MS), and the cytokine engenders permeabilization of in vitro blood-brain barrier (BBB) models. We sought to determine the effect of TWEAK on human brain microvascular endothelial cells (BMECs), specifically the hCMEC/D3 cell line. We used microarray analysis to investigate the transcriptomic changes of hCMEC/D3 induced by rhTWEAK incubation at three timepoints (3h, 12h, and 24h). We identified a set of differentially expressed genes (DEGs) probing the d