Spatial transcriptomics-correlated electron microscopy integrates transcriptional and ultrastructural responses to brain injury
Peter Androvic, Martina Schifferer, Katrin Perez Anderson, Ludovico Cantuti-Castelvetri, Hanyi Jiang, Hao Ji, Lu Liu, Garyfallia Gouna, Stefan Berghoff, Simon Besson-Girard, Johanna Knoferle, Mikael Simons, Ozgun Gokce · FENS Forum 2024
Understanding the complexity of cellular function within a tissue necessitates the combination of multiple phenotypic readouts. Here, we developed a method that links spatially-resolved gene expression of single cells with their ultrastructural morphology by integrating multiplexed error-robust fluorescence in situ hybridization (MERFISH) and large area volume electron microscopy (EM) on adjacent tissue sections. Using this method, we characterized in situ ultrastructural and transcriptional responses of glial cells and infiltrating T-cells after demyelinating brain injury in male mice. We ide
ePoster · Neuroscience