ePoster · Neuroscience
In vitro pain model in cell lines expressing human TRPV1
Yajaira Feller Sánchez, Sahar Avazzadeh, Leo R Quinlan · FENS Forum 2024
Capsaicin (CAP), has undergone extensive research in recent years due to its potential for pain relief, attributed to triggering the activation of TRPV1 receptors, known to be sensitized in painful conditions. This study aims to investigate CAP role in pain therapy within hTRPV1-expressing cell systems, namely ND7/23-hTRPV1 and CHO-hTRPV1. ND7/23 transfection with hTRPV1 was conducted to generate a neuronal model mirroring dorsal root ganglion neurons physiology, involved in pain transmission. To ascertain its specificity to CAP, calcium imaging (CI) was performed. Additionally, automated patc