Unraveling neuroinflammation and cytoskeleton dynamics in brain ischemia: Insights from an oxygen-glucose deprivation model of stroke in organotypic hippocampal cultures for anti-inflammatory strategies
Natalia Bryniarska-Kubiak, Andrzej Kubiak, Ewa Trojan, Małgorzata Lekka, Agnieszka Basta-Kaim · FENS Forum 2024
Ischemic stroke results in neuroinflammation. While several studies addressed its molecular mechanism, we know less about the contribution of physical cues to stroke pathomechanism. In the present work, we aimed to address the significance of nervous tissue cytoskeleton rearrangements and changes in its mechanical properties in neuroinflammation. We applied oxygen-glucose deprivation (OGD) on rat organotypic hippocampal cultures (OHCs) as our model of brain ischemia. We observed that OGD significantly increases LHD and NO release from OHCs. HIF-1α protein expression was also significantly incr
ePoster · Neuroscience