Topic: ischemic brain injury

Background: Microglia activation is an early response in ischemic brain injury, which initiates neuroinflammation. Microglia express Sigma-1 receptors (Sig-1R) at the mitochondria-associated membranes (MAM) of the endoplasmic reticulum(ER), which have been implicated in cellular stress responses. Here we set out to evaluate whether the Sig-1R agonist N,N-dimethyltryptamine (DMT) modulates microglia activation. Materials and methods: Isolated microglia cultures were prepared from the cortex of neonatal Sprague Dawley rats. On day 6, the cells were treated with lipopolysaccharide (LPS;20ng/ml) o