Dual effect of anandamide and its endogenous precursor 20:4-NAPE on DRG neuronal excitability and nociception
Anirban Bhattacharyya, Daniel Vasconcelos, Diana Spicarova, Jiri Palecek · FENS Forum 2024
Aim: Determine the effect of Anandamide (AEA) and its endogenous precursor 20:4 NAPE (n-arachidonoyl phosphatidylethanolamine; NAPE) on neuronal excitability of dorsal root ganglion (DRG) neurons and pain-like behavior using in vitro and in vivo experiments.Methods: Fluorescence imaging (Fura 2) of calcium changes in cultured DRG neurons with repeated high K+ (30 mM) pulses was used as an in vitro model of neuronal excitability. Intrathecal application of drugs in rats and subsequent measurement of thermal (Hargreaves test) and mechanical sensitivity (Von Frey test) was used to assess nocicept
ePoster · Neuroscience