Dose-dependent effect of alpha-synuclein overexpression on neuronal cells
Miraj Ud Din Momand, Karolína Albertusová, Diana Mjartinová, Ľubica Fialová, Dominika Fričová · FENS Forum 2024
In this study, we aim to create a novel approach to understanding the complexity of alpha-synuclein (a-Syn) induced pathological changes at the cellular level in the ReNVM neural progenitor cells, that can readily differentiate into dopaminergic neurons. Additionally, we aim to analyze in greater detail intracellular changes and differences in extracellular signaling induced by a-Syn in a dosage-dependent manner.We created novel neuronal cell models of PD in ReNVM cells background, stably overexpressing GFP-tagged a-Syn either wild type (WT) or its A53T mutant via lentivirus transduction. Cell
ePoster · Neuroscience