Topic: Light Beads Microscopy

[Aims] Neurons in mouse visual cortex encode high-dimensional sensory information and lower-dimensional behavioral variables. Here we asked how behavioral signals are organized across the cortical volume, and whether they follow the organization of visual activity.[Methods] We used Light Beads Microscopy (Demas et al, 2021) to functionally image Ca2+ transients at cellular resolution from large volumes of cortex (3 x 3 x 0.5 mm^3) in awake, headfixed transgenic mice expressing GCaMP6s. To segment individual cells, we developed a novel volumetric cell extraction pipeline by accelerating and ext