Topic: Light Sheet Fluorescence Microscopy

Traditional histological techniques, which require imaging of individual tissue sections, are widely used but tend to be error-prone, time-consuming, and can lead to section loss or non-uniform deformation. Optical sectioning provides a faster and simpler alternative. For instance, light sheet fluorescence microscopy enables the production of high-resolution images of entire brains. This project aimed to use light sheet fluorescence microscopy as a complementary technique to visualize juxtacellular recorded and labeled neurons in anesthetized rats. Our novel approach involved labeling a single

According to the World Health Organization (WHO), a growing number of 0.5 billion people currently suffer from disabling hearing loss (HL). While causes of HL are diverse, it mainly originates in dysfunctions of the inner ear. Recent improvements in light sheet fluorescence microscopy allow nanometric examination of the whole intact cochlea in immunohistochemistry. These findings may help to identify and quantify disease mechanisms on a new scale and aid in the development of new treatment options for patients with severe HL. In this study, we assess the nano-architecture of the cochlea using