Longitudinal single-cell and brain transcriptomic characterization of microglia signatures during experimental demyelination and remyelination
Athena Boutou, Ilias Roufagalas, Katerina Politopoulou, Spyros Tastsoglou, Maya Abouzeid, Giorgos Skoufos, Michael R Johnson, Lesley Probert · FENS Forum 2024
Microglia are key mediators in CNS demyelinating neurodegenerative diseases, controlling the disease environment by inhibiting or favoring CNS repair. Here we combine single-cell RNA-sequencing of cortical microglia and brain bulk RNA-sequencing, together with 3D brain imaging in experimental cuprizone demyelination to provide a longitudinal characterization of brain transcriptome and microglia signatures during disease progression and remyelination. First, brain RNA-sequencing of healthy, demyelination and remyelination states revealed upregulation of microglia genes involved in inflammation,
ePoster · Neuroscience