Topic: marmoset prefrontal cortex

Brain connectomics using large volume electron microscopy (vEM) has been developed in a few neuroscience laboratories. This methodology allows us to analyze synaptic connections on a large scale, such as cortical columnar wiring structures. We remodeled our automated tape collecting ultramicrotome (ATUM) to control the timing of cutting to achieve reliable collection of serial ultrathin sections on slots of a grid tape. These sections were securely and quite reliably placed at similar positions within each grid slot. A transmission EM (TEM) equipped with a high-throughput EM imaging system (Bl