Comparative transcriptome profiling of multiple human induced pluripotent stem cell-derived sensory neuron populations and functional validation of pain targets on automated patch clamp systems
Vincent Truong, Aaron Randolph, Irene Lu, Rita Cerone, Alison Obergrussberger, Rodolfo Haedo, Tim Strassmaier, Patrick Walsh · FENS Forum 2024
Nociceptors are a subset of sensory neurons that relay painful stimuli from the peripheral tissues. Multiple nociceptor subtypes exist and can respond to different thermal, mechanical, or chemical noxious stimuli. Recent findings with transcriptomics have identified gene expression patterns that classify these sub-types by different ion channels including NaV1.7, NaV1.8, NaV1.9, TRPV1, TRPA1, P2X3, HCN and a number of others that have been implicated in pain. In this study, we have identified a method to rapidly generate a novel TRPA1+ human induced pluripotent stem cell (hiPSC)-derived sensor
ePoster · Neuroscience