Methylcobalamin promotes Ras-mediated Akt phosphorylation to induce anti-inflammatory phenotype in macrophages following peripheral nerve injury: A rat model study
Toru Iwahashi, Hiroyuki Tanaka, Toshiki Shimada, Yoshiaki Yoshimura, Katsuyuki Konishi, Atsushi Kamata, Mai Konishi, Arisa Kazui, Ryoya Shiode, Satoshi Miyamura, Kunihiro Oka, Seiji Okada · FENS Forum 2024
In this study, we aimed to explore methylcobalamin (MeCbl), a common treatment for peripheral nerve injuries, and its impact on macrophage phenotypes (inflammatory or anti-inflammatory) at nerve injury sites and the underlying mechanism. Using a sciatic nerve crush injury model in 6-week-old Wistar rats, we administered either saline (control group) or MeCbl (MeCbl group) via osmotic pumps for sustained systemic delivery. We assessed macrophage phenotypes in transverse sections of the sciatic nerve up to 14 days post-surgery using fluorescence immunostaining. In vitro, we induced inflammatory
ePoster · Neuroscience