Chemogenetic inhibition of the lateral hypothalamic area to decrease food intake in rats: A proof-of-concept study and the optimization of technical parameters
Zsolt Kristóf Bali, Tamás Kitka, Péter Kovács, Angelika Bodó, Lili Veronika Nagy, István Hernádi · FENS Forum 2024
Designer receptors exclusively activated by designer drugs (DREADD) are chemogenetic tools that enable the selective activation or silencing of neurons in a given brain area. According to classical findings on the role of the lateral hypothalamic area (LHA) in hunger, we hypothesized that selective silencing of the LHA may decrease food intake.We bilaterally injected adeno-associated viral (AAV) vectors carrying the gene of a modified M4 muscarinic acetylcholine receptor into the LHA of rats. Later, rats were treated with DREADD actuator agents and food intake was measured in a 30 min time-win
ePoster · Neuroscience