Topic: mScarlet3

Fluorescent calcium imaging is an essential tool for measuring cellular activity, particularly as it applies to neurons and reporting action potentials. Genetically encoded calcium indicators are incredibly versatile and particularly useful for both in vivo and in vitro research. Depending on the application and use of indicators, the desired properties can drastically change from being very bright, to a very strong signal, to having fast dynamics, or any combination of the three. However, there are several disadvantages to using existing red shifted calcium indicators, those limitations being