Dissecting the role of autophagy to elucidate the differential response of oligodendrocytes and astrocytes to hypoxic injury in vitro
Sagar Tyagi, Vadanya Shrivastava, Devanjan Dey, JB Sharma, JK Palanichamy, S Sinha, P Seth, S Sen · FENS Forum 2024
Hypoxic injury in developing glial cells and their differential response can result in neurological deficits in neonates. This study attempts to understand the role of autophagy in developing premyelinating oligodendrocytes (pre-OL), mature-OL and astrocytes exposed to hypoxic injury. Human fetal neural stem cell-derived pre-OL, mature OL and astrocytes were exposed to hypoxia (0.2% oxygen) and normoxia (20% oxygen) for 48 hours followed by RNA-sequencing. Transcriptomic analysis revealed upregulation of autophagy in mature OL and astrocytes but not in pre-OL. MO3.13 (resembling pre-OL) and SV
ePoster · Neuroscience