Topic: neurobiotin

Traditional histological techniques, which require imaging of individual tissue sections, are widely used but tend to be error-prone, time-consuming, and can lead to section loss or non-uniform deformation. Optical sectioning provides a faster and simpler alternative. For instance, light sheet fluorescence microscopy enables the production of high-resolution images of entire brains. This project aimed to use light sheet fluorescence microscopy as a complementary technique to visualize juxtacellular recorded and labeled neurons in anesthetized rats. Our novel approach involved labeling a single