It takes two - or three: Comparing human pluripotent stem cell derived glia-neuron co-cultures to neuron monoculture under basal and injury conditions
Jin-Yuan Wang, Jeanne Chan, Kasandra McCormack, Allen C. Eaves, Sharon A. Louis, Erin Knock · FENS Forum 2024
Current advances allow for efficient generation of neuron, astrocyte and microglia cultures from human pluripotent stem cells (hPSCs). However, monocultures lack physiologically important glia-neuron interactions. We created a protocol to co-culture neurons with astrocytes and microglia to assess the impact of glia on neuronal morphology and recovery under injury conditions. We generated hPSC-derived forebrain neurons, astrocytes and microglia using the STEMdiff™ Forebrain Neuron, Astrocyte and Microglia culture systems, respectively. We maintained neuron-astrocyte co-cultures for one week usi
ePoster · Neuroscience