Topic: neuronal lineages

Morphogen gradients play a critical role in directing cell fate specification throughout development but are not yet implemented by current in vitro models. Human induced pluripotent stem cell derived brain organoids recapitulate early cellular and molecular mechanisms of brain development. Because they lack spatial organization to fully mimic the in vivo topographical organization, we introduce a new method to model early neural tube patterning by exposing organoids to continuous gradients within a five-chamber mesofluidic device. We hypothesized that the introduction of oriented signaling ce