Topic: NF-κB signalling pathway

This study investigates the anti-inflammatory effects of ibrutinib in microglia inflammation induced by lipopolysaccharide (LPS) .To investigate the effects of ibrutinib on oxidative stress and inflammation in mouse microglial C8-B4 cell line, cells were treated with ibrutinib (1 and 10 μM) or vehicle (1% DMSO) for 1 h, followed by 1 μg/ml LPS or PBS in case of the control samples, for 23 h. Intracellular ROS was measured by using fluorescence microscopy using 2′,7′-dihydrofluorescein diacetate dye (DCFH-DA). Inflammatory mediator levels (NF kappa beta, TLR4, TNFalpha), and anti-inflammatory c