Human iPSC-derived neurogenin 2 (NGN2) cortical neurons develop functional connectivity and small-world network topology in vitro
Mahsa Khayat-Khoei, Nazanin Ershadinia, David Oluigbo, Timothy P H Sit, Susanna Mierau · FENS Forum 2024
Microelectrode array (MEA) recordings from human induced pluripotent stem cell (iPSC)-derived neurons show disease-related phenotypes including differences in firing and burst rates. To reveal microscale network activity, we investigated age-related differences in the functional connectivity and network topology. We cultured human neurogenin 2 (NGN2) iPSC-derived neurons with human astrocytes on 64-electrode MEAs (n=15 cultures). We performed 10-minute recordings weekly from days-in-vitro (DIV) 14-35 and analysis with MEA-NAP (https://github.com/SAND-Lab/MEA-NAP/). We applied one-way ANOVA and
ePoster · Neuroscience