Topic: nine neuronal subtypes

Current capabilities of state-of-the-art in vivo imaging during freely moving behavior can record two, spectrally distinct, fluorophores. This severely limits the number of cell types identifiable in a functional imaging experiment. Here we present a pipeline that enables the distinction of nine neuronal subtypes from regions defined by behaviorally relevant cells during in vivo GCaMP imaging. These subtypes are identified utilizing unique fluorophores that are co-expressed with GCaMP, unmixed by multispectral lambda imaging on a confocal microscope, and spectral fingerprints co-registered wit