The role of disulfide bonds in GluN1 in the regulation of the early trafficking and functional properties of GluN1/GluN2 subtypes of NMDA receptors
Jakub Netolicky, Petra Zahumenska, Marharyta Kolcheva, Martin Horak · FENS Forum 2024
AIMS: Investigate the impact of disrupting specific disulfide bonds in the GluN1 subunit on the early trafficking and functional properties of conventional NMDARs.METHODS:HEK293 cells were utilized to express wild-type (WT) or mutant forms of GluN1/GluN2A and GluN1/GluN2B receptors. Surface expression and colocalization of NMDAR with the Golgi apparatus were observed using fluorescence confocal microscopy. Electrophysiological properties of NMDARs were assessed via the whole-cell patch-clamp technique. In addition, NMDA-induced excitotoxicity in rat hippocampal neurons was examined specificall
ePoster · Neuroscience