Metabolic shift towards pentose-phosphate-pathway sustains NOX activity in pro-inflammatory microglia
Nathan Strogulski, Janeen Laabei, Carly Douglas, Sara V. Fraj, Patrick Griffin, Sahil Threja, Gloria Vegliante, David J. Loane · FENS Forum 2024
Elevated NADPH-oxidase (NOX) activity, particularly NOX2, is implicated in the persistent pro-inflammatory phenotype observed in microglia following traumatic brain injury. NOX activation requires metabolic support, especially within the pentose-phosphate-pathway (PPP), to fulfil the heightened NADPH demand. Our objective was to identify key metabolic changes associated with NOX activation during pro-inflammatory stimulation in microglia. Using an immortalized microglia (IMG) cell line and PPP inhibitor, 6-aminonicotinamide (6-AN), we stimulated IMGs with LPS (100 ng/mL) and/or 6-AN for 24 hou
ePoster · Neuroscience