Extraembryonic source of serotonin involved in neurodevelopment
Niccolò Milani, Laura Boreggio, Alexander Mordhorst, Sthefanie C. A Gonçalves, Raisa Brito Santos, Fatimunnisa Qadri, Natalia Alenina, Michael Bader · FENS Forum 2024
This study aims to evaluate the contribution of extraembryonic sources of serotonin to prefrontal cortex (PFC) development and understand the placental role and mechanisms of serotonin supplementation to the foetus. To this end, we used mice deficient in serotonin production (TPH1 and TPH2 KO) and serotonin transporters (SERT and OCT3 KO). We assessed serotonin levels in placenta and embryonic brain during different phases of prenatal neurodevelopment using HPLC and immunohistochemistry. Furthermore, we evaluated the PFC maturation and serotonergic wiring by 3D brain ECi imaging in these mice.
ePoster · Neuroscience