ePoster · Neuroendocrinology
Omentin-1 in the mouse hypothalamic neuronal cells: Expression and effect on the regulation of gonadotropin-releasing hormone expression and secretion, viability, and apoptosis in GT1-7 cells
Natalia Respekta-Długosz, Aleksandra Greggio, Ewa Mlyczyńska, Pascal Froment, Joëlle Dupont, Agnieszka Rak · FENS Forum 2024
Omentin-1 (OMNT1), is adipokine involved in regulation of energy metabolism, insulin sensitivity, and reproduction. Our previous study documented expression and in vitro effect of OMNT1 on endocrine function of porcine anterior pituitary cells. However, its role in hypothalamus remains unknown. The present study examines expression and impact of OMNT1 on the mouse hypothalamic neuronal GT1-7 cells (in vitro model of GnRH-secreting neurons). The mRNA expression of OMNT1 and its potential receptor, insulin receptor, as well as co-localization of OMNT1 with GnRH, were determined using RT-qPCR and