Evaluation of synaptic connectivity and dysfunction in aging mouse brains using an RNAscope multiomic spatial imaging assay (MSIA) that detects RNA, proteins, and protein interactions
Chengxin Zhou, Zhenhua Li, Ji Zhang, Yifan Wang, Pehr Williamson, Ge-Ah Kim, Sonali Deshpande, Miao Yuan, Suganya Chandrababu, Lina Duan, Ching-Wei Chang, Betty Booker, Li-chong Wang, Maithreyan Srinivasan · FENS Forum 2024
Advancements in spatial imaging technologies (SIT) have broadened transcriptomic discovery capacity. Yet, development of orthogonal technologies for follow-up translational studies have trailed. We have developed MSIA that utilizes non-temporal, optical barcodes by combining the advantages of RNAscope™ double-Z probes and Hiplex v2 signal amplification technology.Fresh-frozen and FFPE brain coronal sections were collected from C57BL/6J mice of 3 age groups: 1 month, 6 months, and 12 months, respectively. MSIA was executed in 3 cycles, with 100 RNA targets in cycles 1&2 and then up to 4 pro
ePoster · Neuroscience