ePoster · Neuroscience
RatDISCO, an optical clearing and immunolabelling pipeline for large rat (and mice) tissue
Cristina Martinez Gonzalez, Kirsty Craigie, Sally Till, Britt Van De Gevel, Peter Kind, Nathalie Rochefort, Ian Duguid, Matt Nolan · FENS Forum 2024
Understanding brain function in health and disease requires unbiased three-dimensional mapping of its neurochemical architecture. Traditional histological methods require mechanical tissue sectioning, leading to cutting artefacts, lengthy processing times and loss of crucial three-dimensional information. To overcome that, optical clearing techniques have soared since 3DISCO allowed us to visualise neuronal networks in the whole mouse brain1. Immunolabelling in whole organs is possible; however, antibody penetration is a challenge and most clearing protocols are tailored for mice or human tiss