Topic: paired patch-clamp experiments

The ability to efficiently and reliably estimate the presence and magnitude of cortical synapses is critical to understanding how synaptic connectivity relates to neural computation, behaviour, and disease. Two-photon optogenetic stimulation paired with whole-cell electrophysiology allows for potential presynaptic partners to be tested for a given patched cell by measuring optogenetically evoked postsynaptic currents (PSCs). However, existing optogenetic mapping approaches fail to reach their potential for three critical reasons: (1) they rely on stimulating neurons one at a time, (2) they use