pathoDISCO-HE: Light sheet microscopy and fluorescent labelling of glioblastoma multiforme for 3D virtual H&E imaging and improved pathohistological evaluation
James Oakes-Klein, Saiedeh Saghafi, Adelheid Wöhrer, Georg Widhalm, Hans-Ulrich Dodt · FENS Forum 2024
Traditional histopathological evaluation of gliomas relies on haematoxylin and eosin (HE) staining of sectioned tissue. Whilst this technique allows excellent visualisation of nuclei and cytoplasm alike, there are a number of limitations. For example, only a small number of sections from each case can be analysed meaning that key diagnostic features may be missed. To circumvent this problem we aimed to develop a novel technique for 3D-imaging of large glioblastoma specimens with HE-like labelling.We identified cresyl violet as a suitable fluorescent haematoxylin analogue for nuclei labelling i
ePoster · Neuroscience