Topic: phototoxicity

In-vivo measurements are inherently hampered by the motion of behaving animals, particularly in case of using voltage sensitive dyes when the signal is acquired from small regions of interest to get the maximal temporal precision. Here we present an FPGA-based, clearly acousto-optical 3D online motion correction for signal extraction on high temporal resolution of 100 kHz on a volume of 500x500x400 um up to 100 Hz of motion with a residual motion under 1 um at proper signal-to-noise ratio. The correction can be combined with all scanning modes, including point-scan, squared- and ribbon-based s