Topic: polydimethylsiloxane (PDMS)

Neuronal cultures are an excellent experimental tool to study the collective behaviour of neuronal ensembles in a controlled environment. However, neurons cultured on flat surfaces present limitations in terms of their functionality, as they exhibit a synchronous dynamic behaviour that differs from the much richer repertoire of brain activity [1].In order to address this limitation, we studied the capacity to break the isotropy in connectivity and enrich dynamics by modulating the spatial arrangement of neurons in the substrate they grow. Specifically, we cultured neurons on polydimethylsiloxa