Topic: presynaptic partners

Monocular deprivation (MD), the temporary closure of one eye, shifts neuronal responses in the visual cortex towards the open eye. This functional change is accompanied by formation of new dendritic spines. However, the presynaptic partners of these new spines have not yet been identified. Here we introduce a novel approach, two-timeframe monosynaptic rabies virus tracing, to survey the whole brain for new inputs associated with ocular dominance (OD) plasticity.First, in a tdTomato reporter mouse we infect starter cells (expressing a helper construct) with a rabies virus carrying eGFP and indu