Alpha-synuclein pathology from human-derived Lewy body inoculations in the mouse olfactory bulb: Modelling early Parkinson’s disease
Charmaine Helk-Lim, Lianne Robinson, Bettina Platt, Sanna Janhunen, Gernot Riedel · FENS Forum 2024
We characterized a novel model of prodromal Parkinson’s disease (PD) induced by inoculating human alpha-synuclein into the olfactory bulb to trigger alpha-synucleinopathy in wildtype mice. Inoculums were obtained from post-mortem human temporal cortex tissues by sucrose gradient fractionation. C57Bl/6J male mice were inoculated with Lewy Body (LB)-containing fractions (hsyn+) from patients with idiopathic PD; control-injected mice were inoculated with non-LB PD fractions (hsyn-), aggregated non-toxic fractions from non-PD patients (hCtrl), or the corresponding vehicle (Veh). Mice were evaluate
ePoster · Neuroscience