ePoster · Neuroscience
Hypoxia-inducible factor-2alpha in focal cerebral ischemia
Vincent von Oepen, Joachim Fandrey, Tristan Leu · FENS Forum 2024
Stroke is one of the major reasons for death and disability. Insufficient blood flow in stroke interferes the brain from proper oxygenation, leading to the stabilization of hypoxia-inducible factors (HIFs). To determine the role of HIFs in stroke, particularly HIF-2α, an in vivo model called tMCAO (transient middle cerebral artery occlusion) and an in vitromodel with human SH-SY5Y cells was used. On both, Hif-2α deficient and wild type mice, tMCAO was conducted for two hours. In addition to RNA and protein samples, tissue for immunofluorescence microscopy was collected of ischemic and control